sdf1 α  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: Head and neck dermatitis is exacerbated by Malassezia furfur colonization, skin barrier disruption, and immune dysregulation

doi: 10.3389/fimmu.2023.1114321

Figure Lengend Snippet: Immunohistochemistry of HND and non-HND facial skin specimens. (A) Representative histological image at 100x magnification (left) and 200x magnification (right). Quantified staining intensity of (B) SDF-1α, (C) IL-1β, (D) TGF-β, (E) TNF-α, and (F) VEGF. (Unpaired two-tailed t-test, SDF-1α; p = 0.0014, IL-1β; p = 0.0374, TGF-β; p = 0.0058, TNF-α; p = 0.0035, VEGF; p = 0.0002) HND, head and neck dermatitis; SDF-1 α , stromal cell-derived factor-1-alpha; IL-1 β , Interleukin-1-beta; TGF- β , transforming growth factor-beta; TNF- α , tumor necrosis factor-alpha; VEGF, vascular endothelial growth factor. * : p<0.05; ** : p<0.01.

Article Snippet: Immunohistochemical staining was performed using paraffin-embedded sections with antibodies against factor VIII-related antigen (1:100, ab236284, Abcam), stromal cell-derived factor-1-alpha (SDF1-α) (1:100, ab25117, Abcam, Cambridge, United Kingdom), Interleukin-1-beta (IL-1-β) (1:100, ab2105, Abcam), tumor necrosis factor-alpha (TNF-α) (1:50, ab1793, Abcam), transforming growth factor-beta (TGF-β) (1:100, ab66043, Abcam), and vascular endothelial growth factor (VEGF) (1:200, ab1316, Abcam).

Techniques: Immunohistochemistry, Staining, Two Tailed Test, Derivative Assay

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Retinal Progenitor Cells Exhibit Cadherin-Dependent Chemotaxis across Transplantable Extracellular Matrix of In Vitro Developmental and Adult Models

doi: 10.1155/2023/1381620

Figure Lengend Snippet: Summary of growth factors and extracellular matrix (ECM) used to examine the migratory behaviors of retinal progenitors cultured from Drosophila melanogaster (DPs) and from rodent (MPs).

Article Snippet: Stromal-derived growth factor 1-alpha (SDF1- α ; ThermoFisher Catalog #RP-8658) , 100 ng/mL , MPs.

Techniques: Cell Culture, Concentration Assay

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Retinal Progenitor Cells Exhibit Cadherin-Dependent Chemotaxis across Transplantable Extracellular Matrix of In Vitro Developmental and Adult Models

doi: 10.1155/2023/1381620

Figure Lengend Snippet: Measurement of the chemotaxis of stem-like cells. (a) Numbers of motile Drosophila progenitors (DPs) exposed to concentration gradients of insulin ( N = 12), brain-derived neurotrophic factor (BDNF, N = 12), and pyramus (Pyr, N = 12) normalized to control (media only, N = 12). (b) Numbers of motile Mus progenitors (MPs) in response to concentration gradients of insulin-like growth factor 1 (IGF-1, N = 30), epidermal growth factor (EGF, N = 27), and stromal cell-derived factor 1-alpha (SDF-1 α , N = 28) and normalized to control (media only, N = 30). Bars indicate statistically significant increases compared to control ( ∗ p < 0.05).

Article Snippet: Stromal-derived growth factor 1-alpha (SDF1- α ; ThermoFisher Catalog #RP-8658) , 100 ng/mL , MPs.

Techniques: Chemotaxis Assay, Concentration Assay, Derivative Assay, Control

Journal: Stem Cells International

Article Title: CXCR4 + Sorted Adipose-Derived Stem Cells Enhance Their Functional Benefits and Improve Cardiac Function after Myocardial Infarction

doi: 10.1155/2022/6714765

Figure Lengend Snippet: SDF-1 α was upregulated in infarcted myocardium. (a) SDF-1 α (red) was substantially observed on the vessel wall in infarcted myocardium at 3 and 7 days after myocardial infarction, which was scantly expressed in remote normal myocardium. (b, c) The protein expression of SDF-1 α in infarcted myocardium peaked at 3 days after myocardial infarction and remained elevated at 7 days after myocardial infarction relative to remote normal myocardium. Scale bar represents 50 μ m. ∗ p < .05 versus remote normal myocardium. Abbreviations: SDF-1 α : stromal cell-derived factor-1 α ; MI: myocardial infarction; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: After blocking for 1 hour in 1% BSA PBS buffer, cells were incubated for 1 hour in 0.3% Triton X-100/PBS buffer with primary antibody against SDF-1 α (Abcam, Cambridge, MA, USA).

Techniques: Expressing, Derivative Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Curcumol Undermines SDF-1 α /CXCR4/NF- κ B Signaling Pathway to Suppress the Progression of Chronic Atrophic Gastritis (CAG) and Gastric Cancer

doi: 10.1155/2022/3219001

Figure Lengend Snippet: Curcumitol regulates protein expression in the CAG model. (a) Immunohistochemical detection of NF- κ B, SDF-1 α , and CXCR4 expression. (b) Western blot detection of p-NF- κ B expression. (c) ELISA detection of TNF- α , IL-6 and IL-1 β expression.

Article Snippet: Antibodies used in this study were listed as follows: NF- κ B (1 : 500, 10745-1-AP, Proteintech, China), SDF-1 α (1 : 500, ab155090, Abcam, England), CXCR4 (1 : 200, Ab19035, Abclonal, China), p-NF- κ B (1 : 500, 3033T, CST, USA).

Techniques: Expressing, Immunohistochemical staining, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Curcumol Undermines SDF-1 α /CXCR4/NF- κ B Signaling Pathway to Suppress the Progression of Chronic Atrophic Gastritis (CAG) and Gastric Cancer

doi: 10.1155/2022/3219001

Figure Lengend Snippet: The regulation of curcumol on apoptosis, migration, and invasion of gastric cancer cells. (a) Flow cytometry assay on cellular apoptosis. (b) Transwell detection on gastric cancer cells' migration and invasion. (c) Western blot detection of SDF-1 α , CXCR4, NF- κ B, and p-NF- κ B protein expression in gastric cancer cells.

Article Snippet: Antibodies used in this study were listed as follows: NF- κ B (1 : 500, 10745-1-AP, Proteintech, China), SDF-1 α (1 : 500, ab155090, Abcam, England), CXCR4 (1 : 200, Ab19035, Abclonal, China), p-NF- κ B (1 : 500, 3033T, CST, USA).

Techniques: Migration, Flow Cytometry, Western Blot, Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Curcumol Undermines SDF-1 α /CXCR4/NF- κ B Signaling Pathway to Suppress the Progression of Chronic Atrophic Gastritis (CAG) and Gastric Cancer

doi: 10.1155/2022/3219001

Figure Lengend Snippet: SDF-1 α reverses the inhibitory effect of curcumol on gastric cancer cells. (a) Western blot detection onto SDF-1 α , CXCR4, NF- κ B, and p-NF- κ B protein expression. (b) MTT detection on cellular viability. (c) Flow cytometry detection on apoptosis rate. (d) Transwell detection on gastric cancer cells' migration and invasion.

Article Snippet: Antibodies used in this study were listed as follows: NF- κ B (1 : 500, 10745-1-AP, Proteintech, China), SDF-1 α (1 : 500, ab155090, Abcam, England), CXCR4 (1 : 200, Ab19035, Abclonal, China), p-NF- κ B (1 : 500, 3033T, CST, USA).

Techniques: Western Blot, Expressing, Flow Cytometry, Migration